FastQCFastQC Report
Tue 2 Jan 2018
ERR1629987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28046611
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAATTCATCGTGACGTTTGGCAATGTTATTCCTGTTCCTCTTGGTAT363260.12952010494244742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACC64450.028.83737270
AGAACGC48050.026.03674961
AACGCTC49950.025.06497263
TGCGACG45600.021.86252610
GTATCGA28600.021.377814
CCCGTAT33800.021.178221
CGCCTTA70650.020.69172964
CCCCTAC67900.020.51269369
CGGTTCG47700.019.8093853
TTCGCAC98400.019.7682978
ACGTTAT51700.019.61873214
ACCTTCG49000.019.43393945
GACGTTA52800.019.4075813
TTGCGAC60050.019.2044989
CAGAACG66900.018.95464760
CGTATCG34600.018.7752633
CGACGTT53450.018.65165912
GAACGCT67900.018.37925762
TCGCACT67700.018.3171319
CTACCGT60750.018.06980731
Produced by FastQC (version 0.11.5)