FastQCFastQC Report
Tue 2 Jan 2018
ERR1629987_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629987_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28046611
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCC727850.2595144204766843No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG704170.2510713326469283No Hit
AGCACATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGAC498590.17777192403032221No Hit
AGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGC359610.1282186999349048No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG317790.11330780749232054No Hit
CTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCC304650.10862274946516712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG123750.034.36277
GAACGCT121700.034.2626579
GTCGAAC120650.034.1999339
AACGCTG127250.032.82486310
TCGAACG128350.032.2599640
ACGGTAA131700.031.5266744
AGTCGAA135150.030.60569838
GGCCTAA140400.030.33442523
GAACGGT139150.029.78551142
CGGTAAC139250.029.67021245
GCCTAAC141750.029.48427424
CGAACGG140850.029.35002341
AACGGTA141400.029.28902443
CAAGTCG144450.028.70203436
AAGTCGA150800.027.86493537
TGAACGC160700.026.637258
ATTGAAC168600.026.6181836
ACGCTGG173200.024.47731611
TAACACA174750.024.21711527
GACAGGC231500.024.0048122
Produced by FastQC (version 0.11.5)